A move to Acorn Business Park, in Woodseats, will allow S4S to double in size and provide it with the latest technology and advanced facilities.
Directors Neil Bullement and Matt Everatt believe the company's success during the last two years has been fuelled by the launch of its Smilelign system for aligning teeth.
Neil said: 'Funding from RBS has enabled us to purchase the new site which provides additional security because our current premises are rented.
'The bank have been extremely supportive and enabled us to more than double our work space.'
RBS relationship manager David Fielding said: 'S4S is an impressive local business operating in a niche market.
'They are focused on educating dentists and the medical profession on the effectiveness and use of their products which are all manufactured on site in Sheffield.
'The move will help them to strengthen their workforce and increase their production to meet increased demands for their products and services.'